Automated freeze-thaw cycles for decellularization of tendon tissue - a pilot study
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چکیده
منابع مشابه
Automated freeze-thaw cycles for decellularization of tendon tissue - a pilot study
BACKGROUND Decellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of essential decellularization steps like freeze-thawing is crucial for the development of more standardized decellularization protocols and commercial graft production under good m...
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متن کاملFreeze-thaw decellularization of the trabecular meshwork in an ex vivo eye perfusion model
OBJECTIVE The trabecular meshwork (TM) is the primary substrate of outflow resistance in glaucomatous eyes. Repopulating diseased TM with fresh, functional TM cells might be a viable therapeutic approach. Decellularized TM scaffolds have previously been produced by ablating cells with suicide gene therapy or saponin, which risks incomplete cell removal or dissolution of the extracellular matrix...
متن کاملThe Effects of Multiple Freeze–Thaw Cycles on the Biomechanical Properties of the Human Bone-Patellar Tendon-Bone Allograft
Soft tissue allografts, such as the bone-patellar tendon-bone (BPTB) graft, have been frequently used for anterior cruciate ligament (ACL) reconstruction. As allografts are subjected to freezing and thawing for multiple cycles, the objective of this study was to measure the changes of the biomechanical properties of the human BPTB allograft after 4 and 8 freeze-thaw cycles in comparison to a si...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2017
ISSN: 1472-6750
DOI: 10.1186/s12896-017-0329-6